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Research

Projects

  • 3D electron microscopy reconstruction of proteins:
    • The Kv7 channel in detergent, nanodiscs and lipodiscs: Kv7 (Karlova M.)
    • The Kv10.2 channel in a closed state (Glukhov G.)
    • Nucleosome complexes with RNA polymerase and FACT (Karlova M. and Studitsky V. lab.)
    • Hof1 protein F-BAR complexes with Bnr1 formin, actin and septine filaments (Stanisheva-Konovalova T., Vakhrusheva A.)
    • Arp2/3 complex structure, Tankyrase with Arpinum inactivator (Chemeris A.)
    • Luz27 bacteriophage structure(Sedov A. S.)
    • OBP bacteriophage chaperonin in a free state and with an ATP analogue (Stanisheva-Konovalova T, Wang X.)
    • 2D Dps crystals and the interaction of Dps with DNA in vitro and in vivo (Danilova Y., Moiseenko A.)
    • The study of the stiffness of cells which express mutant vimentin or that lack vimentin (Vakhrusheva A.)
    • ULF structure(vimentine protofilaments) according to cryo-EM (Vakhrusheva A.)

Collaboration

Collaboration

Facilities

FEI Tecnai G2 12 Spirit Twin 120kV
  1. Cryoelectron microscope Tecnai G2 12 Spirit Twin (FEI) (120 kV, cryo holder and cryo station for inserting samples) PDF
  2. Electron microscope JEOL2100 (JEOL) (200 kV, high tilt stage +- 70 degrees)
  3. FEI Eagle 4k CCD camera - field of view: 16 Мpх PDF
  4. Semi-automatic cryo plunger (Vitrobot, FEI) PDF
  5. HPLC (Waters)
  6. LC Akta Purifier (GE Healthcare)
  7. Ettan IPGphor 3 IEF System (GE Healthcare) PDF
  8. High-performance desktop microcentrifuge Beckman Coulter Airfuge (120000 rpm)
  9. High-speed centrifuge Optima L-100XP (Beckman) PDF
  10. Tablet spectrophotometer, fluorometer (Zenith)
  11. Real-time PCR (Bio-Rad iQ5 Multicolor) PDF
  12. Electroporator for eukaryotic and prokaryotic cells (Bio-Rad)
  13. Nikon Super Coolscan 9000ED PDF